南湖新聞網訊（通訊員 胡軍(jun) ）近日，農(nong) 業(ye) 微生物資源發掘與(yu) 利用全國重點實驗室、湖北洪山實驗室晏向華教授課題組在Microbiome雜誌發表題為(wei) “Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs”的研究論文。該研究鑒定了生豬腸道核心優(you) 勢真菌Kazachstania slooffiae，闡明其通過賴氨酸琥珀酰化修飾調控豬腸上皮細胞糖酵解代謝的作用機製，為(wei) 理解生豬腸道優(you) 勢真菌與(yu) 宿主的互作提供依據。
腸道健康是生豬健康養(yang) 殖的前提，腸道微生物廣泛參與(yu) 營養(yang) 物質代謝和免疫調節等重要生理過程，目前發掘生豬腸道內(nei) 原創性功能微生物資源較少，嚴(yan) 重製約了功能微生物在生豬健康養(yang) 殖中的高效利用。因此，深入發掘生豬腸道功能微生物並解析其作用機製對實施腸道菌群幹預改善豬生長與(yu) 健康具有重要理論意義(yi) 和潛在應用價(jia) 值。
生豬腸道內(nei) 除棲息大量細菌外，腸道真菌亦是腸道微生物的重要組成部分，其對宿主生理的重要調控作用逐漸被重視。占核心優(you) 勢的腸道微生物在宿主腸道內(nei) 的豐(feng) 度較高，可與(yu) 宿主形成較為(wei) 穩定的共生關(guan) 係，並參與(yu) 宿主代謝的調控。蛋白質翻譯後修飾對細胞代謝具有重要調控作用，然而腸道真菌對宿主蛋白質翻譯後修飾的作用機製仍不清楚。
我校生豬精準飼養(yang) 團隊綜合運用真菌ITS擴增子測序和宏基因組學技術解析了7個(ge) 品種豬（即杜×（長×大）豬、藏豬、萊蕪豬、沙子嶺豬、從(cong) 江香豬、環江香豬和寧鄉(xiang) 豬），共計56頭斷奶仔豬和56頭育肥末期豬的腸道真菌區係組成。發現不同品種豬的腸道真菌區係有明顯差異，但Kazachstania slooffiae是7個(ge) 品種斷奶仔豬腸道內(nei) 豐(feng) 度均為(wei) 最高的真菌。隨後從(cong) 豬糞便中分離並鑒定了Kazachstania slooffiae，進一步開展了Kazachstania slooffiae與(yu) 豬腸上皮細胞共培養(yang) 的實驗。結果表明Kazachstania slooffiae可顯著降低豬腸道上皮細胞的賴氨酸琥珀酰化修飾水平，且顯著富集在糖酵解通路，進一步研究發現Kazachstania slooffiae通過激活SIRT5活性，降低賴氨酸琥珀酰化修飾水平，進而促進腸道上皮細胞糖酵解活性的作用機製。綜上，本論文係統解析了生豬腸道真菌區係組成，闡明了Kazachstania slooffiae可通過賴氨酸去琥珀酰化修飾促進豬腸上皮細胞糖酵解代謝的作用機製，為(wei) 實施腸道菌群幹預改善生豬腸道上皮細胞的能量代謝提供新思路與(yu) 策略。
星空体育网站入口官网動物科學技術學院、動物醫學院博士後胡軍(jun) 為(wei) 第一作者，晏向華教授為(wei) 通訊作者。本研究得到國家自然科學基金、博士後創新人才計劃、博士後麵上項目的資助。
【英文摘要】
Background
Gut fungi are increasingly recognized as important contributors to host physiology, although most studies have focused on gut bacteria. Post-translational modifications (PTMs) of proteins play vital roles in cell metabolism. However, the contribution of gut fungi to host protein PTMs remains unclear. Mining gut fungi that mediate host protein PTMs and dissecting their mechanism are urgently needed.
Results
We studied the gut fungal communities of 56 weaned piglets and 56 finishing pigs from seven pig breeds using internal transcribed spacer (ITS) gene amplicon sequencing and metagenomics. The results showed that Kazachstania slooffiae was the most abundant gut fungal species in the seven breeds of weaned piglets. Kazachstania slooffiae decreased intestinal epithelial lysine succinylation levels, and these proteins were especially enriched in the glycolysis pathway. We demonstrated that K. slooffiae promoted intestinal epithelial glycolysis by decreasing lysine succinylation by activating sirtuin 5 (SIRT5). Furthermore, Kazachstania slooffiae-derived 5′-methylthioadenosine metabolite promoted the SIRT5 activity.
Conclusions
These findings provide a landscape of gut fungal communities of pigs and suggest that Kazachstania slooffiae plays a crucial role in intestinal glycolysis metabolism through lysine desuccinylation. Our data also suggest a potential protective strategy for pigs with an insufficient intestinal energy supply.
：
審核人：晏向華
