（通訊員 莫全）近日，學院李家奎教授團隊在journal of hazardous material發表了題為(wei) “Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens”的研究論文，該研究通過轉錄組、蛋白組與(yu) 代謝組聯合分析，驗證了肉雞脛骨軟骨生長板ECM重塑在肉雞脛骨軟骨發育不良的重要影響，同時發現TD肉雞胰島功能紊亂(luan) 引起的血糖升高是促發軟骨細胞ECM過度重塑的重要原因。該研究為(wei) 家禽脛骨軟骨發育不良的機製解析和藥物防治奠定了堅實的理論基礎。
肉雞脛骨軟骨發育不良(Tibial Dyschondroplasia，TD)是快速生長肉雞的常見多發性骨骼疾病，也是危害肉雞養(yang) 殖的重要疾病。該病臨(lin) 床表現為(wei) 運動障礙、骨骼畸形、生長性能下降、易於(yu) 發生骨髓炎，屠宰加工中容易骨折導致胴體(ti) 品質下降。TD的發生嚴(yan) 重影響雞肉品質、造成家禽養(yang) 殖行業(ye) 大量經濟損失。李家奎教授團隊多年來一直專(zhuan) 注於(yu) 肉雞脛骨軟骨發育不良的發病機製解析和藥物防治研究，前期相關(guan) 成果已在《Ecotoxicology and Environmental Safety》《Phytomedicine》和《poultry science》發表。本次研究以AA肉雞作為(wei) 研究材料建立福美雙誘導的TD模型，對TD肉雞和健康肉雞的軟骨生長板進行了轉錄組，蛋白質組和代謝組測序。通過關(guan) 聯分析發現TD發生過程中其生長板軟骨細胞糖酵解功能顯著下降，細胞外基質ECM合成途徑顯著增強，此外檢測分析發現肉雞發生TD涉及腸道菌群，胰島功能和血糖代謝紊亂(luan) ，且通過體(ti) 內(nei) 體(ti) 外試驗證明了肉雞血糖代謝紊亂(luan) 是促進軟骨細胞ECM過度分泌，導致TD肉雞脛骨生長板增厚，引起跛行的重要原因。本研究結果為(wei) 肉雞TD發生機製提供了新的理論見解，也為(wei) TD的防治和治療提供了新的思路。
TD肉雞脛骨生長板蛋白質組學分析
發病TD肉雞脛骨生長板潛在代謝通路
我院在讀博士生莫全為(wei) 論文第一作者。李家奎教授為(wei) 論文通訊作者。該研究工作得到了國家自然科學基金項目的資助。
論文鏈接：
審核：李家奎
【英文摘要】
Pesticide thiram is widely used in agriculture and has been demonstrated to cause tibial dyschondroplasia (TD) in birds. However, the underlying mechanism remains unclear. This work used multi-omics analysis to evaluate the molecular pathways of TD in broilers that were exposed to low level of thiram. Integrative analysis of transcriptomic, proteomic, and metabolomic revealed thiram activity in enhancing pathological ECM remodeling via attenuating the glycolysis pathway and activating the hexosamine and glucuronic acid pathways. Intriguingly, we found hyperglycemia as a crucial factor for ECM overproduction, which resulted in the development of TD. We further demonstrated that high glucose levels are caused by islet secretion dysfunction in thiram-treated broilers. A combination of factors, including lipid disorder, low-grade inflammation, and gut flora disturbance, might contribute to the dysregulation of insulin secretion. The current work revealed the underlying toxicological mechanisms of thiram-induced tibial dyschondroplasia through blood glucose disorder via the gut-pancreas axis in chickens for the first time, which makes it easier to figure out the health risks of pesticides for worldwide policy decisions.
